IOT-based process control prevents methanol toxicity

In cooperation with the Institute of Applied Microbiology (iAMB), RWTH Aachen University, Innovation Centre, Corbion (The Netherlands).

In recent years, the methylotrophic yeast Ogataea polymorpha has established itself as an extremely valuable production organism for heterologous protein expression. Its unique metabolism enables the efficient utilisation of methanol both as a carbon source and as an inducer for the strong AOX promoter system. However, fermentation process control with methanol poses a considerable challenge, as even small excess concentrations have a toxic effect on the cells, while undersupply can inhibit growth and limit product formation.

With the fermSYS Buddy a direct, IoT-based coupling of VOC sensors and pump actuators enables dynamic control of the methanol supply, consistently maintaining concentrations within the optimal range.

This approach effectively circumvents

1. the problematic early toxicity of methanol, optimises the substrate uptake rate of the cells and results in a significant increase in cell density and product yield.

2. the core weakness of manual data protocols –> their lack of real-time responsiveness.

Growth and methanol concentration with manual addition of methanol

Growth and methanol concentration with continuos monitoring and control of methanol

Thanks to automation with the fermSYS Buddy, we gain a new level of independence in the laboratory through continuous IoT monitoring. This freedom allows us to focus on what is essential – the interpretation and availability of data - regardless of location -, the planning of new experiments and the creative solution of complex scientific questions.

The simplicity of use, in particular the absence of complex software installations or extensive modifications to the existing fermenter control system, makes the solution particularly practical and suitable for widespread use in biotechnological production environments.

Lorenzo García Tejada (M.Sc), Prof. Dr. Lars Blank in the laboratory of the Institute of Applied Microbiology (iAMB) at RWTH Aachen University, Corbion Innovation Centre (Netherlands)

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